View Full Version : Post LBK Neolithic remains from Poland

17-10-16, 21:08
They're talking about Middle Neolithic



Investigating kinship of Neolithic post-LBK human remains from Krusza Zamkowa, Poland using ancient DNA, Anna Juras et al
"We applied an interdisciplinary approach to investigate kinship patterns and funerary practices during the middle Neolithic. Genetic studies, radiocarbon dating, and taphonomic analyses were used to examine two grave clusters from Krusza Zamkowa, Poland. To reconstruct kinship and determine biological sex, we extracted DNA from bones and teeth, analyzed mitochondrial genomes and nuclear SNPs using the HID-Ion AmpliSeq™ Identity panel generated on Illumina and Ion Torrent platforms, respectively. We further dated the material (AMS14C) and to exclude aquatic radiocarbon reservoir effects, measures of carbon and nitrogen stable isotopes for diet reconstruction were used. We found distinct mitochondrial genomes belonging to haplogroups U5b2a1a, K1c and H3d in the first grave cluster, and excluded maternal kin patterns among the three analyzed individuals. In the second grave cluster one individual belonged to K1a4. However, we could not affiliate the second individual to a certain haplogroup due to the fragmented state of the mitochondrial genome. Although the individuals from the second grave cluster differ at position 6643, we believe that more data is needed to fully resolve this issue. We retrieved between 26 and 77 autosomal SNPs from three of the individuals. Based on kinship estimations, taking into account the allelic dropout distribution, we could not exclude first degree kin relation between the two individuals from the second grave cluster. We could, however, exclude a first degree kinship between these two individuals and an individual from the first grave cluster. Presumably, not only biological kinship, but also social relations played an important role in the funerary practice during this time period. We further conclude that the HID-Ion AmpliSeq™ Identity Panel may prove useful for first degree kin relation studies for samples with good DNA preservation, and that mitochondrial genome capture enrichment is a powerful tool for excluding direct maternal relationship in ancient individuals."

I don't know if there's enough for real autosomal analysis.

A mix of U5b, K, and H3 seems about right for a Middle Neolithic samples, yes?

Isn't K1a4 one of the so called founding mtDna lines for Ashkenazim?

17-10-16, 21:23
Nice to have more ancient mtDNA. I will add them now to my ancient DNA page (http://www.eupedia.com/europe/ancient_european_dna.shtml), which I have been re-vamping for better legibility this week, calculating frequencies for all Neolithic cultures with more than 30 samples.

All these lineages have been found in Neolithic before, except K1c, which was in Mesolithic Greece, and also came back later with Steppe invasions.

Isn't K1a4 one of the so called founding mtDna lines for Ashkenazim?

Nope. The main Jewish K subclades are K1a1b1a, K1a9, and K2a2a. There are other rarer subclades like K1a7, K1a8 and K2c.

K1a4 was found in Neolithic Anatolia, Cardial Spain, LBK Alsace and in the Salzmünde groupe of the Funnelbeaker culture. It's found all over Europe nowadays.