My take on this is that people want to deal with facts and try to stay with that the Academic research indicates with respect to DNA and genetic relationships. I use all these amateur calculators just like anyone else. However, I have never relied 100% on any one of them. I always run as many of them as possible and see who the amateur calculator results converge with the published DNA literature. So I just used Eurogenes K13 Ancient, which in the past has not been the best for Southern Europeans, particularly Greeks, Southern Italians (including Sicilians). However, I must say the Ancient samples now in the K13 Ancient is really impressive. So I did just 2 runs. The first I just used source data from Anatolian Neolithic (the Source for EEF) and Bronze Age and Neolithic Age Lazio Samples from Antonio et al 2019. So just with the source data below, I can get a 4 way admixture model and distance of 2.7.
Bar31_Anatolia_Early_Neolithic_,16.28,0.00,41.45,0.00,34.40,6.14,0.00,0.00,0.00,0.00,0.00,0.44,1.29
Bar8_Anatolia_N.SG_8071_ybp,10.93,0.00,45.07,0.00,40.17,3.82,0.00,0.00,0.00,0.00,0.00,0.00,0.00
Bon002_Anatolia_N_Boncuklu.SG_10078_ybp,17.64,0.40,44.91,0.00,33.21,2.73,0.00,0.00,0.00,0.00,1.12,0.00,0.00
Boncuklu002,20.71,0.00,42.81,0.00,30.75,4.24,0.00,0.00,0.00,0.00,1.01,0.00,0.48
I0707_Anatolia_N_8092_ybp,7.33,0.00,46.18,0.00,41.13,5.35,0.00,0.00,0.00,0.00,0.00,0.00,0.00
I0708_Anatolia_N_8097_ybp,7.25,0.00,43.57,0.08,44.29,4.81,0.00,0.00,0.00,0.00,0.00,0.00,0.00
I0709_Anatolia_N_8086_ybp,8.75,0.00,46.20,0.00,40.49,4.57,0.00,0.00,0.00,0.00,0.00,0.00,0.00
I0723_Anatolia_N_7870_ybp,6.40,0.00,47.49,0.00,41.94,4.11,0.00,0.00,0.00,0.00,0.07,0.00,0.00
I0724_Anatolia_N_7950_ybp,12.29,0.00,47.43,0.00,35.97,4.05,0.00,0.00,0.26,0.00,0.00,0.00,0.00
I0726_Anatolia_N_7950_ybp,9.55,0.00,46.27,0.00,37.57,6.60,0.00,0.00,0.00,0.00,0.00,0.00,0.00
I0727_Anatolia_N_7950_ybp,1.40,0.00,51.31,0.00,41.58,5.70,0.00,0.00,0.00,0.00,0.00,0.00,0.00
I0736_Anatolia_N_8300_ybp,9.05,0.00,43.92,0.00,42.32,4.71,0.00,0.00,0.00,0.00,0.00,0.00,0.00
I0744_Anatolia_N_8273_ybp,7.26,0.00,47.78,0.00,42.05,2.91,0.00,0.00,0.00,0.00,0.00,0.00,0.00
I0745_Anatolia_N_8251_ybp,10.49,0.00,42.35,0.00,39.56,7.54,0.00,0.00,0.00,0.00,0.06,0.00,0.00
I1096_Anatolia_N_8300_ybp,10.55,0.00,43.86,0.00,41.95,3.64,0.00,0.00,0.00,0.00,0.00,0.00,0.00
I1097_Anatolia_N_8288_ybp,5.09,0.00,47.04,0.00,44.06,3.82,0.00,0.00,0.00,0.00,0.00,0.00,0.00
I1098_Anatolia_N_8288_ybp,8.97,0.00,40.63,0.00,45.08,5.32,0.00,0.00,0.00,0.00,0.00,0.00,0.00
I1099_Anatolia_N_8300_ybp,11.33,0.00,44.13,0.00,41.04,3.50,0.00,0.00,0.00,0.00,0.00,0.00,0.00
I1100_Anatolia_N_8300_ybp,6.96,0.00,48.01,0.00,40.77,3.84,0.00,0.00,0.00,0.00,0.42,0.00,0.00
I1101_Anatolia_N_8300_ybp,10.27,0.00,43.37,0.00,42.30,4.06,0.00,0.00,0.00,0.00,0.00,0.00,0.00
I1102_Anatolia_N_8300_ybp,11.70,0.00,45.89,0.00,36.40,5.80,0.00,0.00,0.00,0.00,0.11,0.00,0.10
I1103_Anatolia_N_8300_ybp,5.63,0.00,46.90,0.00,39.72,7.71,0.00,0.00,0.00,0.00,0.04,0.00,0.00
I1580_Anatolia_N_8195_ybp,7.32,0.00,47.45,0.00,38.61,6.62,0.00,0.00,0.00,0.00,0.00,0.00,0.00
I1581_Anatolia_N_8254_ybp,6.99,0.00,48.39,0.00,38.11,6.51,0.00,0.00,0.00,0.00,0.00,0.00,0.00
I1583_Anatolia_N_8281_ybp,4.17,0.00,48.75,0.00,41.73,5.35,0.00,0.00,0.00,0.00,0.00,0.00,0.00
I1584_Anatolia_C_5776_ybp,9.90,0.00,24.65,25.27,35.62,2.63,1.46,0.00,0.48,0.00,0.00,0.00,0.00
Tep002_Anatolia_N_Tepecik_Ciftlik.SG_8585_ybp,7.21,0.00,41.68,0.00,43.08,8.04,0.00,0.00,0.00,0.00,0.00,0.00,0.00
Tep002_Tepecik_Ciftlik_level_5_c_6500_BCE_8500_BP_,12.96,0.00,33.37,1.13,39.75,9.07,0.00,0.00,0.44,0.00,0.00,2.71,0.54
Tep003_Anatolia_N_Tepecik_Ciftlik.SG_8505_ybp,4.96,0.00,32.53,11.24,45.49,5.78,0.00,0.00,0.00,0.00,0.00,0.00,0.00
Tep004_Anatolia_N_Tepecik_Ciftlik.SG_8295_ybp,6.08,0.00,34.12,0.84,45.53,13.43,0.00,0.00,0.00,0.00,0.00,0.00,0.00
Tepecik002_Tepecik002_,12.96,0.00,33.37,1.13,39.75,9.07,0.00,0.00,0.44,0.00,0.00,2.71,0.54
R10_Abruzzo_Neolithic_Farmer_Italy,14.30,0.00,46.36,0.00,34.52,4.82,0.00,0.00,0.00,0.00,0.00,0.00,0.00
R2_Abruzzo_Neolithic_Farmer_Italy,13.37,0.00,49.33,0.00,33.67,3.63,0.00,0.00,0.00,0.00,0.00,0.00,0.00
R3_Abruzzo_Neolithic_Farmer_Italy,12.78,0.00,48.34,0.00,34.59,4.29,0.00,0.00,0.00,0.00,0.00,0.00,0.00
R8_Abruzzo_Neolithic_Farmer_Italy,15.43,0.00,46.33,0.00,35.46,2.78,0.00,0.00,0.00,0.00,0.00,0.00,0.00
R6_Abruzzo_Neolithic_Farmer_Italy,32.18,0.88,53.51,0.00,11.47,1.79,0.00,0.00,0.13,0.00,0.04,0.00,0.00
R9_Abruzzo_Neolithic_Farmer_Italy,10.08,0.00,46.86,0.00,38.39,4.67,0.00,0.00,0.00,0.00,0.00,0.00,0.00
R1_Abruzzo_Teramo_Late_Bronze_Age_Italy,28.96,13.59,21.58,9.88,19.66,3.43,0.00,0.00,1.43,0.99,0.48,0.00,0.00
Target: PalermoTrapani
Distance: 2.7970% / 2.79697573 | R6P | ADC: 0.25x RC | |
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45.0 | R1_Abruzzo_Teramo_Late_Bronze_Age_Italy |
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29.0 | I1584_Anatolia_C_5776_ybp |
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13.5 | Tep002_Tepecik_Ciftlik_level_5_c_6500_BCE_8500_BP_ |
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12.5 | Tepecik002_Tepecik002_ |
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If I use all 1,784 ancient Samples in the K13 Ancient, I can get a distance of 0.6742
Target: PalermoTrapani
Distance: 0.6742% / 0.67422881 | ADC: 0.25x RC
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54.1 | SZ40_Hungary_Langobard_1442_ybp |
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14.8 | R131_Lazio_Rome_Roman_Imperial |
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14.3 | I9033_Mycenaean_1352_bc_M_ |
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7.1 | R1283_Lazio_Rome_Medieval_Italy |
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7.1 | R35_Lazio_Rome_Italy_Late_Antiquity |
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2.6 | R132_Lazio_Rome_Roman_Imperial |
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So again, these various calculators to me are sensitive to the source samples included (do they capture ancestries for different populations accurately, or Not?), the estimation technique used (Statistical estimation), etc.