According to the Gladstone DNA Project, the lineage of the famous Victorian British Prime Minister William Ewart Gladstone (1809-1898) belongs to R1b-L21. Indeed, his genealogy can be traced back to James Gledstanes who died in 1493 in Lanarkshire, Scotland.

Gladstone is considered by some one of the three greatest Liberals in British history. In a career lasting over sixty years, during the Victorian Golden Age of Great Britain, he served as Prime Minister four separate times (1868–1874, 1880–1885, February–July 1886 and 1892–1894), more than any other person. He is remembered for introducing secret ballot at elections, for proposing Irish Home rule and improving the legal rights of Irish tenant farmers.