The old South Thracian samples are still in the top rank ANF-region and some had, on top of that, more Anatolian-related admixture than others.
We can also check that with other confirmed Thracian samples we have, like this female:
Ancient DNA sample I5769 from Bulgaria. Bulgaria_Dzhulyunitsa_IA
www.exploreyourdna.com
She (I5769) is from North of the Balkan/Haemus mountains, Dzhulyunitsa (Veliko Tarnovo province, Lyaskovets municipality), and has lower non-European and more steppe also. If I plot here and do the usual admixture run, she ends up closer with the 2 Northern male individuals.
At the same time with another individual from Rozovo, South Thrace, Bulgaria_LIA.AG:I19500.AG__BC_250__Cov_38.87%,0.118376,0.161469,0.001131,-0.057494,0.020927,-0.011156,-0.00329,-0.003231,-0.001432,0.031891,0.002436,0.009292,-0.01665,0.003028,-0.019815,-0.02201,0.000391,0.005954,0.012821,-0.005253,-0.006114,0.008656,-0.003574,0.006748,-0.010538
His coverage is not great, he is from the Late Iron Age and he was assigned to E-BY14160, which also shows an Aegean-Anatolian pull, just like the three I singled out from the Old South Thracian set. The branch he has E-BY14160 is clearly South Thracian oriented and was later found in Byzantine Anatolia and modern Turks also, having branches in the Near East, being overall as Southern or more Southern than E-BY5022.
The crucial point here is, the Aegean-Anatolian backflow is more likely to be stronger in South of the Haemus/Balkan mountains than North of it. And we might just see that. Here is the PCA to illustrate the pattern:
Nearly all samples in the current set with such a South Eastern pull and less steppe/HG ancestry have Southern branches like E-BY5022, E-BY14160 and E-Y19509. Even the most ANF-rich samples from E-FGC11457, E-L241, E-Y3183 and E-CTS9320 in the set are still with the Northern oriented two males, the North Bulgarian female ("Thracian") and still more Northern than them.
And I don't think that's by chance, because again, the chances for such a South Eastern pull in the earlier Thracian period, before the Roman admixture, which sticks out if being big in most instances, are much greater South of the Balkan mountains - it fits with sites like Kapitan Andreevo and Rozovo.
You can also see the very pure (practically no HG, practically no Iranian) E-CTS2001 sample (I44144) which just like I46263 being pulled towards Salcuta/Salcuta-Bodrogkeresztur/w. steppe admixture. But only there, directly down, not to the right.
On the other hand, the late Thracian from Rozovo being primarily pulled to the right, towards the Aegean-Anatolian.
This kind of proves the suspicion that the most Southern Thracian fringe received some sort of Aegean-Anatolian admixture EXTRA which the original Thracian core likely didn't have. Because even the two typical males from the same site don't have it (as much) and the same is true for the female from North of the Balkan mountains. Again, these are all well-documented samples and the new batch of E-V13 individuals with Thracian-like autosomals just proves the point, because they all appear less admixed and more Northern - even without clear traces of non-Thracian admixture!
And even more important: Those which are probably even more pure and Southern pulled, being pulled towards Salcuta/Vinca/Barcin, pure ANF, NOT Tepecik-Çiftlik-like ancestry, like the outliers in the South East!
This tells us the high ANF was typically Proto-Thracian, but the increased Tepecik-Çiftlik-like admixture was quite likely not, but picked up in the South East first. And the Kapitan Andreevo samples show the process, because some indivdiuals (male Thracian lineages) are still rather unmixed/less mixed, while especially the R-Z93 and the female are already more heavily shifted.
Especially in the later periods, this is likely to separate unmixed North from South Thracians. In the North Thracians, more Celtic-like, steppe-like, Kyjatice-like and Illyrian-like (in the West) admixture will be more typical. Just going by the high density cluster of both the new and old samples, one might ask whether that's the Daco-Thracian core profile:
In all directions from it, relevant samples thin out.
Of the Vekerzug individuals, one of the South Eastern shifted ones is clearly in that circle, the other barely so and from Thracian Hallstatt Kartal only one individual makes it, Kartal 1 (UKR001)
Ancient DNA sample saag_UKR001 from Ukraine. Ukraine_Odesa_ThracianHallstatt_EIA
www.exploreyourdna.com
UKR007, the E-V13, doesn't make it, but he gets close and looks just a bit mixed with some more HG/steppe rich source.
Ancient DNA sample saag_UKR007 from Ukraine. Ukraine_Odesa_ThracianHallstatt_EIA
www.exploreyourdna.com
The other two East Thracians (MJ-12, UKR002) are completely out of range.
On a sidenote, the haplogroups other than E-V13 most commonly associated with the Thracian-type ancestry appear to be R-P312/L2 (Bell Beaker? Tumulus culture?) and R-Z2109. There are a lot of R-Z2109 samples all over the place and we can't date many of the samples either, but that's just what's popping up. The connection to R-P312 and R-Z2109 is in any case stronger than to J-L283, which is something worth being pointed out, regardless of how old than pairing is in many sets.