The Romano et al study mentioned up thread dates from 2003 and used 9 autosomal STR's. For mtDNA analysis it was unable to type below the level of "H", "J" etc., doubtless because it was using Torroni et al. (1996,1998) and references therein. There is no yDNA analysis. Oh, and the most recent computer program used is thirteen years old, an aeon in software and population genetics terms.
With that said, the following is found in the study:
"Fig. 3a shows two clear clusters: Castellammare, Sciacca and Butera which group together 86% of the times and the remaining samples whose structure is, however, less defined. Ragusa and Piazza Armerina are associated 70% of the times, but the evolutionary model suggested by the tree is probably not valid for Troina and Caccamo, which join the tree with bootstrap values of less than 50%. The addition of the mtDNA haplogroup frequencies does not help to resolve the matter: Troina joins Castellammare and Sciacca 62% of times, but Caccamo joins Troina, Castellammare and Sciacca 48% of times, and other combinations of samples in lower percentages.
The maximum likelihood tree obtained by adding three samples from North-Africa (Algeria and Egypt) and the Middle East (Turkey) provides further information: 54% of times Castellammare, Butera and Sciacca are associated with the Middle East sample, while the remaining samples (Troina, Caccamo, Piazza Armerina and Ragusa) are associated with the two samples from North-Africa."
It should be kept in mind that based on only 9 autosomal markers, DNA-Tribes told some northern Europeans that they were North Africans. Although, to be fair, even on a very limited number of markers they placed me precisely in northwestern Tuscany, which represents half of my ancestry. As a recent paper has indicated, however, parts of central and northern Italy do indeed show some significant sub-structure.
The paper does caution, however:
"Establishing a one-to-one correspondence between the genetic (gene and genotypic) heterogeneity of Sicily observed today and a presumed geneticcomposition of its pre-Roman settlers is a very dangerous exercise until one has typed ancient DNA from pre-Roman Sicilian fossils in the relevant archaeological areas...The peopling of Sicily, as very briefly described above, should have caused genetic differentiation on the west-east axis of the island: old classical genetic markers , surnames (Guglielmino et al. 1991) Guglielmino et al.1991, and dialect isoglosses (Ruffino 1997) agree by showing this differentiation. The genetic analysis by Rickards et al. (1998) failed to find this geographical pattern, but our results in
Fig. 2 show that at least the fraction of genetic variability summarized by the most important principal component of our data (which is
26%) is correlated with longitude much more than with latitude.
They conclude with the following statement: "A more reliable association of these diachronic genetic strata to different historical populations (e.g.
Sicani, Elymi, Siculi), if possible, must be postponed to the analysis of more samples and hopefully more informative uniparental DNA markers such as the recently available DHPLC-SNP polymorphisms of the Y chromosome."
Taken as a whole, and most especially because of the dated methods used, I personally don't find this old study dispositive.
That's not to say, of course, that there isn't any population structure within Sicily, especially along an east/west axis. It's just that this study doesn't prove it, in my opinion. Hopefully, in the future, something like it will be attempted again using current programs.